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Imaging and X-Irradiation

A new RTSF Core Facility located in the Biomedical and Physical Sciences Building is now open to MSU Researchers. The X-RAD 320 is located in 1114 BPS and the IVIS Spectrum is located in 1117 BPS.

INSTRUMENTS

X-RAD 320
The X-RAD 320 is a self-contained x-ray system for delivering radiation dosages safely and effectively to specimens e.g. cell cultures, small animals. The X-RAD 320 is located in room 1114 Biomedical Physical Sciences (BPS) Building. Entry is by authorized card access only. All users are required to have completed the ORCBS online class entitled Analytical X-ray Training and submitted a System Training request Form and System Access Form to xrad320@cns.msu.edu prior to being trained to use the machine. You must have a DPPS assigned 4 digit PIN to access the room. An IACUC approved AUF is required for all small animal irradiation studies.

Forms

X-RAD 320 Access

X-RAD 320 Training Request

Protocols

Operating Instructions

Pricing

Service

Price

Initial Training

$72

X-RAD 320

$12/15 min
(billed in 15 min increments)

 
IVIS Spectrum

The IVIS Spectrum is a light tight imaging chamber with integrated gas anesthesia and heated stage for quantitative bioluminescent and fluorescent imaging in vivo and in vitro. Take advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region using the IVIS Spectrums narrow band excitation and emission filters and spectral un-mixing algorithms.

Forms

IVIS Spectrum Imaging Request

IVIS Imaging Access

Pricing

Service

Price

IVIS Imaging

$40/hr
(billed in 30 min increments)

Protocols

Cleaning the IVIS Spectrum

Entry and PPE Requirement

Living Image Software User's Manual

XIC-3 Isolation Chamber - Care and Maintenance

XIC-3 Isolation Chamber - use with IVIS Spectrum

XGI-8 Gas Anesthesia System

XGI-8 Anesthesia System - Quick Reference

XGI-8 Anesthesia Machine - Vaporizer

Technical Reference Material

Spectrum 202: Advanced Imaging presentation

Biology Tech Notes:

Kinetics Curve

Imaging Protocols

Intraperitoneal Injections

Adaptive Fluorescence Background Subtraction


Auto-Exposure

Determine Saturation

Bioluminescence Tomography

Setup and Sequence Acquisition

Topography

Source Reconstruction and Analysis

Drawing ROIs

Subtracting Background ROI from Sequence

Subject ROIs

Fluorescence Tomography

Setup and Sequence Acquisition

Topography

Source Reconstruction and Analysis

Acquisition of High Resolution Images

Image Math

Image Overlay - 2D

Image Overlay - 3D

Imaging Wizard

Load as Group

Spectral Unmixing

Transillumination Fluorescence

Transillumination Fluorescence - Raster Scan

Transillumination Fluorescence - Normalized

Well Plate Quantification

Sandra O'Reilly, PhD
2199 Biomedical Phys Sci
Office Phones: 884-5172 oreillys@cns.msu.edu

Supplies to be provided by Researcher:

Ear tagging equipment (ear punch or tags)

Calipers for tumor measurement

D-Luciferin for Bioluminescent imaging

All needles and syringes

Alcohol pads

2x Gibeck IsogardĀ  HEPA filter ref: 28012. For use with XIC-3 isolation chamber.

Pens, markers and lab notebooks

Flash drive to download data

 
Supplies available from facility:

IVIS cleaning supplies: Sporicidin, MilliQ water, 70% Ethanol

Kim Wipes

Isoflurane (with bottle key adapters)

Oxygen tanks for the XGI-8 anesthesia machine and LEI Medical table top anesthesia machine

Black Artagain paper

F-Air canisters

Sharps container

For the XIC-3 Animal Isolation Chamber: optical lens solution, lint free optical wipes

Germicidal Wipes

For IVIS Spectrum information,
e-mail: ivis@cns.msu.edu

For X-RAD 320 information, e-mail: xrad320@cns.msu.edu